ABSTRACT

Androgenesis is the newest and most secure method to obtain haploid plants in vitro, wherevegetative or generative nucleus of a pollen grain is stimulated to develop into a haploid individual.There are different possibilities for regeneration and formation of microspores in various genotypesof tobacco. In this case, the level of androgenesis was investigated in three tobacco genotypes. NNmedia was used as a basic medium for microspores development and MS-media for rhizogenesis and organogenesis, together with adequate combinations of plant hormones (JAA, BAP, adenine, glutamine and kinetine).